Diffraction Limit PSF
 
Author: Bob Dougherty 
Installation: Download  Diffraction_Limit_PSF.class or Diffraction_Limit_PSF.java to the plugins folder.   (Windows users right-click to download.)  Requires ImageJ version 1.32c or later.
Description: Plugin to compute the point spread function of a diffraction-limited microscope.   See http://yakko.bme.virginia.edu/phy506/   An informal derivation of the Fraunhofer diffraction pattern for this case is provided here: Diffraction-PSF.pdf.
Disclaimer: This code only represents a classical diffraction limit.  If possible, direct measurements of your microscope's psf should be made, or data should be obtained from the manufacturer.
Usage: Fill in the form and compute the psf.  A pop-up window gives the Rayleigh resolution in pixels before the psf is created.   The dB option helps to see the Airy rings, but should usually not be selected if the psf will be used in convolution or deconvolution codes.  An exception is Iterative Deconvolution, which can accept dB inputs.

Versions: 0: 5/25/2004
License:

Copyright (c) 2004-2005, OptiNav, Inc.
All rights reserved.

Redistribution and use in source and binary forms, with or without modification, are permitted provided that the following conditions are met:

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