Generalized Polarization Analysis
 
Authors: Bob Dougherty (rpd@optinav.com)  Orderd by Jesper Søndergaard Hansen.
Installation: Download Generalized_Polarization_Analysis.class to the Plugins folder and restart ImageJ. Source: Generalized_Polarization_Analysis.java
Description: ImageJ plugin to process an RGB image to calculate the generalized polarization function: Intensity(Blue) - Intensity(Green) / Intensity(Blue) + Intensity(Green). See, for example,
Parasassi, T., G. De Stasio, A. d'Ubaldo, and E. Gratton. 1990. Phase fluctuation in phospholipid membranes revealed by Laurdan fluorescence. Biophys. J. 57:1179-1186.
The plugin includes carefully controlled LUTS for the output and the ability to calibrate the image as specified by the customer, Jesper Søndergaard Hansen, in G_factor_calibration_of_GP_microscopy.pdf
Sample:
Input image
Dialog
Histogram output
GPA output
History: November 2010: Ordered
December 2010: Initial versions
August 12, 2011: Calibration feature added
License:

Copyright (c) 2010, 2011 OptiNav, Inc.
All rights reserved.

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